Retention time – time among sample injection and the maximum peak signal of your analyte inside of a chromatogram Know your column: Columns have different pore sizes and solvent tolerances. Look at With all the company from the column to forestall loading incompatible samples and solvents.

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Slim-bore columns (one–two mm) are useful for apps when far more sensitivity is sought after either with Distinctive UV-vis detectors, fluorescence detection or with other detection approaches like liquid chromatography-mass spectrometry Tandem liquid chromatography tactics utiliz

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